Ingabe usebenza emkhakheni wocwaningo lwezokwelapha? Uma kunjalo, kungenzeka ukuthi uke wezwa nge-Sulfo-NHS. Njengoba indima ebalulekile yale nhlanganisela ocwaningweni iqhubeka nokuqashelwa, le nhlanganisela ingena emalabhorethri amaningi emhlabeni jikelele. Kulesi sihloko, sixoxa ngokuthi i-Sulfo-NHS iyini nokuthi kungani iyithuluzi eliwusizo kangaka kulabo abafunda isayensi yezinto eziphilayo.
Okokuqala, iyini i-Sulfo-NHS? Igama lifushane kancane, ngakho ake silichaze. I-Sulfo imele i-sulfonic acid kanti i-NHS imele i-N-hydroxysuccinimide. Uma lezi zinhlanganisela ezimbili zihlangana,I-Sulfo-NHSkukhiqizwa. Le nhlanganisela inezindlela eziningana zokusetshenziswa ocwaningweni lwezokwelapha, kodwa esinye sezimpawu zayo ezibalulekile yikhono lokukhetha amalebula amaprotheni.
I-Sulfo-NHS isebenza ngokusabela nama-amine ayinhloko (okungukuthi amaqembu e-NH2) emaketangeni aseceleni ezinsalela ze-lysine kumaprotheni. Empeleni, i-Sulfo-NHS ihlanganisa amaprotheni “ama-tag”, okwenza kube lula ukuwabona nokuwahlaziya ezivivinyweni ezahlukahlukene. Lokhu kuholele ekutheni izindawo eziningi zocwaningo zikwazi ukuqhubekela phambili ngokunemba okukhulu kanye namazinga aphezulu emininingwane.
Ngakho-ke, i-Sulfo-NHS isetshenziselwani? Ukusetshenziswa okuvamile kwale nhlanganisela kusekucwaningweni kwe-immunology. I-Sulfo-NHS iboniswe ukuthi ifaka amalebula amasosha omzimba nama-antigen ngempumelelo, ivula izindlela ezintsha zokufunda ngezinkinga nezifo zesistimu yomzimba. Ngaphezu kwalokho,I-Sulfo-NHSingasetshenziswa ezifundweni zokusebenzisana kwamaprotheni njengoba ivumela abacwaningi ukuthi babone ngokushesha nangokulula lapho amaprotheni amabili ehlangana khona.
Enye indawo lapho i-Sulfo-NHS isetshenziswa khona kakhulu yileya ye-proteomics. I-Proteomics ifunda isakhiwo kanye nomsebenzi wawo wonke amaprotheni emzimbeni, kanyeI-Sulfo-NHSiyithuluzi elibalulekile kulokhu kuhlaziya. Ngokumaka amaprotheni nge-Sulfo-NHS, abacwaningi bangenza izivivinyo ukuze bathole ulwazi oluningiliziwe mayelana ne-proteome yesidalwa esithile, okungasiza ekuboneni ama-biomarker angaba khona esifo.
I-Sulfo-NHS nayo idlala indima ekuthuthukisweni kwemithi emisha. Lapho abacwaningi bezama ukuthuthukisa umuthi omusha, kubalulekile ukuqinisekisa ukuthi uhlose iphrotheni ehlosiwe hhayi enye iphrotheni emzimbeni. NgokusebenzisaI-Sulfo-NHSukuze bakhethe amathegi amaprotheni, abacwaningi bangabona izinhloso eziqondile zemithi engaba khona, okungasiza ekusheshiseni inqubo yokuthuthukiswa kwemithi.
Ngakho-ke nansi! I-Sulfo-NHS kungenzeka ukuthi ayilona igama elaziwayo ngaphandle komphakathi wesayensi, kodwa le nhlanganisela isheshe iba ithuluzi eliwusizo ocwaningweni lwezokwelapha. Kusukela ocwaningweni lwe-immunology kuya ku-proteomics kuya ekuthuthukisweni kwemithi, i-Sulfo-NHS isiza abacwaningi ukuthi bathuthuke kakhulu kulezi zindawo futhi sijabule ukubona ukuthi yikuphi ukutholwa okulandelayo.
Isikhathi sokuthunyelwe: Juni-12-2023