Ingabe usebenza emkhakheni wocwaningo lwe-biomedical? Uma kunjalo, kungenzeka ukuthi uzwile nge-Sulfo-NHS. Njengoba indima ebalulekile yale nhlanganisela ocwaningweni iqhubeka nokuqashelwa, le nhlanganisela ingena ezindaweni zokucwaninga eziningi emhlabeni jikelele. Kulesi sihloko, sidingida ukuthi iyini iSulfo-NHS nokuthi kungani iyithuluzi elibaluleke kangaka kulabo abafunda isayensi yezinto eziphilayo.
Okokuqala, yini i-Sulfo-NHS? Igama linomoya omude kancane, ngakho-ke masilihlukanise. I-Sulfo imele i-sulfonic acid futhi i-NHS imele i-N-hydroxysuccinimide. Lapho lezi zinhlanganisela ezimbili zihlangana,I-Sulfo-NHSkukhiqizwa. Le nhlanganisela inokusetshenziswa okuningana ocwaningweni lwe-biomedical, kodwa enye yezindawo zayo ezibalulekile yikhono lokukhetha ukukhetha amaprotheni.
I-Sulfo-NHS isebenza ngokusabela ngama-amine ayinhloko (okungukuthi -NH2 amaqembu) emaketangeni aseceleni ezinsalela ze-lysine kumaprotheni. Empeleni, i-Sulfo-NHS ihlanganisa amaprotheni “amathegi,” okwenza kube lula ukuhlonza nokuhlaziya ekuhloleni okuhlukahlukene. Lokhu kuholele ekutheni izindawo eziningi zocwaningo zikwazi ukuqhubekela phambili ngokunemba okukhulu kanye namazinga aphezulu emininingwane.
Ngakho, iSulfo-NHS isetshenziselwa ini? Okunye okuvamile ukusetshenziswa kwalesi sithako ocwaningweni lwe-immunology. I-Sulfo-NHS ikhonjiswe ukuthi ilebula ngempumelelo amasosha omzimba nama-antigen, ivula izindlela ezintsha zokucwaninga ngokuphazamiseka kwesistimu yomzimba nezifo. Ngaphezu kwalokho,I-Sulfo-NHSingasetshenziswa ocwaningweni lokusebenzisana kwamaprotheni-amaprotheni njengoba ivumela abacwaningi ukuthi babone ngokushesha futhi kalula lapho amaprotheni amabili esebenzisana.
Enye indawo lapho i-Sulfo-NHS isetshenziswa khona kakhulu yileyo ye-proteomics. I-Proteomics ifunda ukwakheka nokusebenza kwawo wonke amaprotheni entweni ephilayo, futhiI-Sulfo-NHSiyithuluzi elibalulekile kulokhu kuhlaziya. Ngokumaka amaprotheni nge-Sulfo-NHS, abacwaningi bangenza izivivinyo ukuze bathole ulwazi oluningiliziwe mayelana ne-proteome yento ephilayo, engasiza ekuhlonzeni izimpawu zezinto eziphilayo ezingaba khona zesifo.
I-Sulfo-NHS nayo idlala indima ekuthuthukisweni kwemithi emisha. Uma abacwaningi bezama ukwenza umuthi omusha, kubalulekile ukwenza isiqiniseko sokuthi uqondise kuphrotheni ehlosiwe hhayi enye iphrotheni emzimbeni. NgokusebenzisaI-Sulfo-NHSukuze ukhethe ngokukhetha amaprotheni, abacwaningi bangakwazi ukubona okuqondiwe ngqo kwezidakamizwa ezingase zibe khona, ezingasiza ukusheshisa inqubo yokuthuthukiswa kwezidakamizwa.
Ngakho-ke usunakho! I-Sulfo-NHS ingase ingabi igama elaziwa kakhulu ngaphandle komphakathi wesayensi, kodwa le nhlanganisela iba ithuluzi elibalulekile ocwaningweni lwe-biomedical ngokushesha. Kusukela ocwaningweni lwe-immunology kuye ku-proteomics kuya ekuthuthukisweni kwezidakamizwa, i-Sulfo-NHS isiza abacwaningi ukuthi benze inqubekelaphambili enkulu kulezi zindawo futhi sijabule ukubona ukuthi yikuphi okutholakele okulandelayo.
Isikhathi sokuthumela: Jun-12-2023