Ngaba usebenza kwicandelo lophando lwezonyango? Ukuba kunjalo, kusenokwenzeka ukuba ukhe weva ngeSulfo-NHS. Njengoko indima ebalulekileyo yale khompawundi kuphando iqhubeka nokuqatshelwa, le khompawundi ingena kwiilabhoratri ezininzi kwihlabathi liphela. Kweli nqaku, sixoxa ngokuba yintoni iSulfo-NHS kwaye kutheni isisixhobo esixabisekileyo kwabo bafunda isayensi yebhayoloji.
Okokuqala, yintoni iSulfo-NHS? Eli gama lithetha into ende, ngoko masilichaze. I-Sulfo imele i-sulfonic acid kwaye i-NHS imele i-N-hydroxysuccinimide. Xa ezi zinto zimbini zidibene,I-Sulfo-NHSiveliswa. Le khompawundi ineendlela ezininzi ezisetyenziswa kuphando lwezonyango, kodwa enye yeempawu zayo eziphambili kukukwazi ukulebhelisha iiproteni ngokukhetha.
I-Sulfo-NHS isebenza ngokuphendula ngee-amine eziphambili (oko kukuthi amaqela e-NH2) kwimixokelelwane esecaleni yeentsalela ze-lysine kwiiproteni. Ngokuyintloko, i-Sulfo-NHS idibanisa iiproteni "ezithegiweyo", okwenza kube lula ukuzichonga nokuhlalutya kwiindidi ezahlukeneyo zovavanyo. Oku kuye kwabangela ukuba iindawo ezininzi zophando zikwazi ukuqhubela phambili ngokuchanekileyo okukhulu kunye namanqanaba aphezulu eenkcukacha.
Ngoko ke, isetyenziselwa ntoni iSulfo-NHS? Enye indlela eqhelekileyo yokusebenzisa le khompawundi kuphando lwe-immunology. I-Sulfo-NHS ibonakalisiwe ukuba ibhala kakuhle ii-antibodies kunye nee-antigens, ivula iindlela ezintsha zokufunda ngeengxaki zenkqubo yomzimba yokuzikhusela kunye nezifo. Ukongeza,I-Sulfo-NHSingasetyenziswa kwizifundo zokusebenzisana kweeproteni neeproteni njengoko ivumela abaphandi ukuba bachonge ngokukhawuleza nangokulula xa iiproteni ezimbini zidibana.
Enye indawo apho i-Sulfo-NHS isetyenziswa kakhulu yileyo ye-proteomics. I-Proteomics ifunda ulwakhiwo kunye nomsebenzi wazo zonke iiproteni kwindalo, kwayeI-Sulfo-NHSsisixhobo esibalulekileyo kolu hlalutyo. Ngokubeka iiproteni kwi-Sulfo-NHS, abaphandi banokwenza iimvavanyo ukuze bafumane ulwazi oluthe kratya malunga ne-proteome yesidalwa esithile, nto leyo enokunceda ekuchongeni ii-biomarkers ezinokubakho zesifo.
I-Sulfo-NHS ikwadlala indima ekuphuhlisweni kwamayeza amatsha. Xa abaphandi bezama ukuvelisa iyeza elitsha, kubalulekile ukuqinisekisa ukuba lijolise kwiproteni ecetywayo hayi kwenye iproteni emzimbeni. NgokusebenzisaI-Sulfo-NHSUkuze bakhethe iiproteni, abaphandi banokuchonga iithagethi ezichanekileyo zamayeza anokubakho, nto leyo enokunceda ukukhawulezisa inkqubo yokuphuhliswa kwamayeza.
Ngoko ke nantsi into oyaziyo! I-Sulfo-NHS isenokungabi ligama elaziwayo ngaphandle koluntu lwezenzululwazi, kodwa le nto ikhula ngokukhawuleza ibe sisixhobo esibalulekileyo kuphando lwezonyango. Ukusuka kuphando lwe-immunology ukuya kwi-proteomics ukuya kuphuhliso lwamayeza, i-Sulfo-NHS inceda abaphandi ukuba benze inkqubela phambili enkulu kwezi ndawo kwaye sonwabile ukubona ukuba zeziphi izinto eziza kufunyanwa.
Ixesha lokuthumela: Juni-12-2023