Ngaba usebenza kwinkalo yophando lwebhayoloji? Ukuba kunjalo, usenokuba uvile ngeSulfo-NHS. Njengoko indima ebalulekileyo yale khompawundi kuphando iqhubeka nokubonwa, le khompawundi ingena kwiilebhu ezininzi kwihlabathi jikelele. Kweli nqaku, sixoxa ukuba yintoni iSulfo-NHS kwaye kutheni isisixhobo esibaluleke kangaka kwabo bafunda isayensi yebhayoloji.
Okokuqala, yintoni iSulfo-NHS? Igama linomoya omde, ngoko ke masiliqhekeze. I-Sulfo imele i-sulfonic acid kwaye i-NHS imele i-N-hydroxysuccinimide. Xa ezi zimbini zidibanisa,Sulfo-NHSiveliswa. Le khompawundi inosetyenziso oluninzi kuphando lwebhayoloji, kodwa enye yeempawu zayo eziphambili kukukwazi ukukhetha iiproteni.
I-Sulfo-NHS isebenza ngokuphendula ngee-amine eziphambili (oko kukuthi -amaqela e-NH2) kumakhonkco ecala leentsalela ze-lysine kwiiprotheni. Ngokusisiseko, i-Sulfo-NHS idibanisa "ithegi" yeeprotheni, okwenza kube lula ukuchonga nokuhlalutya kwiimvavanyo ezahlukeneyo. Oku kubangele ukuba iindawo ezininzi zophando zikwazi ukuqhubela phambili ngokuchaneka okukhulu kunye namanqanaba aphezulu eenkcukacha.
Ke, iSulfo-NHS isetyenziselwa ntoni? Olunye usetyenziso oluqhelekileyo lwale khompawundi kuphando lwe-immunology. I-Sulfo-NHS ibonakaliswe ukuba ibhale ngokufanelekileyo ii-antibodies kunye ne-antigens, ivula iindlela ezintsha zokufunda ukuphazamiseka kwe-immune system kunye nezifo. Ukongeza,Sulfo-NHSinokusetyenziswa kwizifundo zokusebenzisana kweeprotheni-protein njengoko ivumela abaphandi ukuba babone ngokukhawuleza kwaye babone xa iiprotheni ezimbini zisebenzisana.
Enye indawo apho iSulfo-NHS isetyenziswa kakhulu yileyo yeproteomics. Iiproteomics zifunda ubume kunye nomsebenzi wazo zonke iiprotheyini kwi-organism, kunyeSulfo-NHSsisixhobo esiphambili kolu hlalutyo. Ngokumaka iiproteni ngeSulfo-NHS, abaphandi banokwenza iimfuniselo ukuze bafumane ulwazi oluthe kratya malunga neproteome yezinto eziphilayo, enokuthi emva koko incede ekuchongeni ii-biomarkers zesifo.
I-Sulfo-NHS ikwadlala indima ekuphuhliseni amayeza amatsha. Xa abaphandi bezama ukuphuhlisa iyeza elitsha, kubalulekile ukuqinisekisa ukuba lijolise kwiprotheyini ekujoliswe kuyo kwaye hayi nayiphi na enye iprotheni emzimbeni. NgokusebenzisaSulfo-NHSukukhetha iiprotheyini, abaphandi banokuchonga iithagethi ezichanekileyo zamachiza anokubakho, anokunceda ukukhawulezisa inkqubo yophuhliso lwamachiza.
Ngoko ke unayo! I-Sulfo-NHS isenokungabi ligama elaziwa kakuhle ngaphandle koluntu lwenzululwazi, kodwa le khompawundi ikhawuleza ibe sisixhobo esibalulekileyo kuphando lwebhayoloji. Ukusuka kuphando lwe-immunology ukuya kwi-proteomics ukuya kuphuhliso lweziyobisi, i-Sulfo-NHS inceda abaphandi ukuba benze inkqubela phambili kule mimandla kwaye siyavuya ukubona ukuba yintoni efunyenweyo ezayo.
Ixesha lokuposa: Jun-12-2023