Unoshanda here muchikamu chekutsvaga mishonga yekurapa? Kana zvakadaro, saka ungave wakanzwa nezveSulfo-NHS. Sezvo basa rakakosha rechikamu ichi mukutsvagisa richiri kuzivikanwa, chikamu ichi chiri kupinda mumarabhoritari mazhinji pasi rose. Muchinyorwa chino, tinokurukura kuti Sulfo-NHS chii uye kuti nei iri chishandiso chakakosha kune avo vanodzidza sainzi yebhayoloji.
Kutanga, chii chinonzi Sulfo-NHS? Zita racho rakapfupikiswa zvishoma, saka ngatiritsanangure. Sulfo inomiririra sulfonic acid uye NHS inomiririra N-hydroxysuccinimide. Kana mishonga iyi miviri yakabatana,Sulfo-NHSinogadzirwa. Mushonga uyu une mashandisirwo akasiyana-siyana mukutsvagisa kwezvekurapa, asi chimwe chezvinhu zvikuru zvawo kugona kunyora mazita emapuroteni.
Sulfo-NHS inoshanda nekuita nemaamine ekutanga (kureva mapoka e-NH2) pamativi emaketani eLysine residues mumapuroteni. Chaizvoizvo, Sulfo-NHS inobatanidza mapuroteni e "tag", zvichiita kuti zvive nyore kuona nekuongorora mukuyedza kwakasiyana-siyana. Izvi zvakaita kuti nzvimbo dzakawanda dzekutsvagisa dzikwanise kufambira mberi nekururama kwakanyanya uye mazinga epamusoro eruzivo.
Saka, Sulfo-NHS inoshandiswa chii? Imwe nzira yakajairika yekushandisa mushonga uyu ndeyekutsvagisa nezvekudzivirira muviri. Sulfo-NHS yakaratidzwa kuti inonyora ma antibodies nema antigen zvinobudirira, ichivhura nzira itsva dzekudzidza nezvezvirwere nezvirwere zvemuviri. Pamusoro pezvo,Sulfo-NHSinogona kushandiswa muzvidzidzo zvekudyidzana kwemapuroteni nemapuroteni sezvo ichibvumira vaongorori kuziva nekukurumidza uye zviri nyore apo mapuroteni maviri anosangana.
Imwe nzvimbo inoshandiswa zvakanyanya neSulfo-NHS ndeye proteomics. Proteomics inodzidza maumbirwo nemashandiro emapuroteni ese ari muchisikwa, uyeSulfo-NHSchishandiso chakakosha mukuongorora uku. Nekuisa mapuroteni muSulfo-NHS, vaongorori vanogona kuita kuyedza kuti vawane ruzivo rwakadzama nezveproteome yechipenyu, izvo zvinogona kubatsira kuona zviratidzo zvinogona kukonzera chirwere.
Sulfo-NHS ine basawo mukugadzirwa kwemishonga mitsva. Kana vaongorori vari kuedza kugadzira mushonga mutsva, zvakakosha kuve nechokwadi chekuti unonanga puroteni yaunoda kwete chero imwe puroteni mumuviri. NekushandisaSulfo-NHSKuti vasarudze mapuroteni, vaongorori vanogona kuona zvinangwa chaizvo zvemishonga inogona kushandiswa, izvo zvinogona kubatsira kukurumidzisa kugadzirwa kwemishonga.
Saka hezvo! Sulfo-NHS ingasave izwi rinozivikanwa kunze kwesainzi, asi chinhu ichi chiri kukurumidza kuva chishandiso chakakosha mukutsvagisa kwezvekurapa. Kubva pakutsvagisa kwezvirwere zvemuviri kusvika kumaproteomics kusvika pakugadzirwa kwemishonga, Sulfo-NHS iri kubatsira vaongorori kufambira mberi kukuru munzvimbo idzi uye tinofara kuona kuti ndezvipi zvichawanikwa zvinotevera.
Nguva yekutumira: Chikumi-12-2023