Ese ukora mu rwego rw'ubushakashatsi ku buvuzi bw'ibinyabuzima? Niba ari byo, ushobora kuba warumvise ibya Sulfo-NHS. Kubera ko uruhare runini rw'iyi mvange mu bushakashatsi rukomeje kumenyekana, iyi mvange irimo kwinjira muri laboratwari nyinshi hirya no hino ku isi. Muri iyi nkuru, turaganira ku cyo Sulfo-NHS ari cyo n'impamvu ari igikoresho cy'agaciro ku biga siyansi y'ibinyabuzima.
Ubwa mbere, Sulfo-NHS ni iki? Izina ryayo rirambuye gato, reka tubisobanure. Sulfo isobanura aside sulfonic naho NHS isobanura N-hydroxysuccinimide. Iyo ibi bintu bibiri bihuye,Sulfo-NHSirakorwa. Iyi mvange ifite akamaro kenshi mu bushakashatsi ku buvuzi bw'ibinyabuzima, ariko kimwe mu bintu by'ingenzi ifite ni ubushobozi bwo gushyira poroteyine mu byiciro bitandukanye.
Sulfo-NHS ikora ikoresheje amine z’ibanze (ni ukuvuga amatsinda ya -NH2) ku ruhande rw’ibisigazwa bya lysine muri poroteyine. Mu by’ukuri, Sulfo-NHS ivanga poroteyine za "tag", bigatuma zoroha kuzimenya no kuzisesengura mu bushakashatsi butandukanye. Ibi byatumye ubushakashatsi bwinshi bushobora gutera imbere neza cyane kandi butanga ibisobanuro birambuye.
None se, Sulfo-NHS ikoreshwa iki? Imwe mu mikoreshereze isanzwe y'iki kintu ni mu bushakashatsi ku budahangarwa bw'umubiri. Sulfo-NHS byagaragaye ko ishyira mu byiciro neza abasirikari b'umubiri na antigens, igatanga inzira nshya zo kwiga ku ndwara n'uburwayi bw'ubudahangarwa bw'umubiri. Byongeye kandi,Sulfo-NHSishobora gukoreshwa mu bushakashatsi ku mikoranire ya poroteyine na poroteyine kuko ituma abashakashatsi bamenya vuba kandi byoroshye igihe poroteyine ebyiri zihurira.
Ikindi gice Sulfo-NHS ikoreshwa cyane ni proteinomics. Proteomics yiga imiterere n'imikorere ya poroteyine zose mu kinyabuzima, kandiSulfo-NHSni igikoresho cy'ingenzi muri ubu busesenguzi. Mu gushyira poroteyine mu byiciro bya Sulfo-NHS, abashakashatsi bashobora gukora igerageza kugira ngo babone amakuru arambuye ku miterere y'umubiri runaka, ibyo bikaba byafasha kumenya ibimenyetso bishobora gutuma habaho indwara.
Sulfo-NHS kandi igira uruhare mu iterambere ry'imiti mishya. Iyo abashakashatsi bagerageza gukora umuti mushya, ni ngombwa kumenya neza ko ureba poroteyine igenewe aho kwibanda ku yindi poroteyine iyo ari yo yose mu mubiri. UkoreshejeSulfo-NHSKugira ngo bashyireho poroteyine mu buryo bwihariye, abashakashatsi bashobora kumenya intego nyazo z'imiti ishobora gukoreshwa, ibi bikaba byafasha kwihutisha igikorwa cyo gukora imiti.
Ngaho rero! Sulfo-NHS ishobora kuba atari ijambo rizwi cyane hanze y’umuryango w’abahanga, ariko iki kintu kirimo kuba igikoresho cy’agaciro mu bushakashatsi ku buvuzi bw’ibinyabuzima. Kuva ku bushakashatsi ku budahangarwa bw’umubiri kugeza ku buvuzi bw’imiti, Sulfo-NHS irimo gufasha abashakashatsi gutera imbere cyane muri izi nzego kandi twishimiye kureba ibizaba nyuma yaho.
Igihe cyo kohereza: Kamena-12-2023