Sulfo-NHS: Sayansi yomwe ili kumbuyo kwa gawo lake lofunika kwambiri mu kafukufuku wa zamankhwala

Kodi mumagwira ntchito yofufuza za sayansi ya zamoyo? Ngati ndi choncho, ndiye kuti mwina munamvapo za Sulfo-NHS. Pamene ntchito yofunika kwambiri ya mankhwala amenewa ikupitilizidwa kudziwika, mankhwala amenewa akulowa m'ma laboratories ambiri padziko lonse lapansi. Munkhaniyi, tikukambirana za Sulfo-NHS ndi chiyani komanso chifukwa chake ndi chida chamtengo wapatali kwa iwo omwe amaphunzira sayansi ya zamoyo.

Choyamba, kodi Sulfo-NHS ndi chiyani? Dzinali ndi lalitali pang'ono, choncho tiyeni tilifotokoze mwachidule. Sulfo imayimira sulfonic acid ndipo NHS imayimira N-hydroxysuccinimide. Pamene mankhwala awiriwa akuphatikizana,Sulfo-NHSimapangidwa. Chomerachi chimagwiritsidwa ntchito kangapo mu kafukufuku wa zamankhwala, koma chimodzi mwa zinthu zake zazikulu ndi kuthekera kolemba ma protein mosankha.

Sulfo-NHS imagwira ntchito pochita zinthu ndi ma amine oyambira (monga magulu a -NH2) omwe ali m'mbali mwa ma lysine residues mu mapuloteni. Kwenikweni, Sulfo-NHS imapanga mapuloteni otchedwa "tag", zomwe zimapangitsa kuti azizindikirike mosavuta ndikuzisanthula m'mayesero osiyanasiyana. Izi zapangitsa kuti madera ambiri ofufuza athe kupita patsogolo molondola kwambiri komanso mwatsatanetsatane.

Ndiye, kodi Sulfo-NHS imagwiritsidwa ntchito chiyani? Chimodzi mwa zinthu zomwe zimagwiritsidwa ntchito kwambiri ndi kafukufuku wa chitetezo chamthupi. Sulfo-NHS yawonetsedwa kuti imagwiritsa ntchito bwino ma antibodies ndi ma antigen, ndikutsegula njira zatsopano zophunzirira matenda ndi matenda a chitetezo chamthupi. Kuphatikiza apo,Sulfo-NHSingagwiritsidwe ntchito mu kafukufuku wokhudzana ndi mapuloteni chifukwa zimathandiza ofufuza kuzindikira mwachangu komanso mosavuta pamene mapuloteni awiri amagwirizana.

Gawo lina lomwe Sulfo-NHS imagwiritsidwa ntchito kwambiri ndi la ma proteomics. Ma proteomics amaphunzira kapangidwe ndi ntchito ya mapuloteni onse m'thupi, ndipoSulfo-NHSndi chida chofunikira kwambiri pakuwunikaku. Polemba ma protein ndi Sulfo-NHS, ofufuza amatha kuchita zoyeserera kuti apeze zambiri zokhudza proteome ya chamoyo china, zomwe zingathandize kuzindikira zizindikiro zomwe zingayambitse matenda.

Sulfo-NHS imathandizanso pakupanga mankhwala atsopano. Ofufuza akamayesa kupanga mankhwala atsopano, ndikofunikira kuonetsetsa kuti akuyang'ana puloteni yomwe ikufuna osati puloteni ina iliyonse m'thupi. Pogwiritsa ntchitoSulfo-NHSPofuna kusankha ma tag a mapuloteni, ofufuza amatha kuzindikira zolinga zenizeni za mankhwala omwe angakhalepo, zomwe zingathandize kufulumizitsa njira yopangira mankhwala.

Ndiye mwamvetsa! Sulfo-NHS mwina si dzina lodziwika bwino kunja kwa gulu la asayansi, koma mankhwalawa akukhala chida chofunikira kwambiri pa kafukufuku wa zamankhwala. Kuyambira kafukufuku wa chitetezo chamthupi mpaka ma proteomics mpaka kupanga mankhwala, Sulfo-NHS ikuthandiza ofufuza kupita patsogolo kwambiri m'magawo awa ndipo tikusangalala kuona zomwe zikubwera mtsogolo.