Koj puas ua haujlwm hauv kev tshawb fawb txog biomedical? Yog tias yog, ces tej zaum koj yuav tau hnov txog Sulfo-NHS. Raws li lub luag haujlwm tseem ceeb ntawm cov tshuaj no hauv kev tshawb fawb tseem raug lees paub, cov tshuaj no nkag mus rau ntau lub chaw soj nstuam thoob ntiaj teb. Hauv tsab xov xwm no, peb tham txog Sulfo-NHS yog dab tsi thiab vim li cas nws thiaj li yog ib qho cuab yeej muaj txiaj ntsig zoo rau cov neeg kawm txog kev tshawb fawb txog tsiaj txhu.
Ua ntej, Sulfo-NHS yog dab tsi? Lub npe ntev me ntsis, yog li cia peb piav qhia me ntsis. Sulfo sawv cev rau sulfonic acid thiab NHS sawv cev rau N-hydroxysuccinimide. Thaum ob lub tshuaj no sib xyaw ua ke,Sulfo-NHSyog tsim tawm. Cov tshuaj no muaj ntau yam kev siv hauv kev tshawb fawb biomedical, tab sis ib qho ntawm nws cov khoom tseem ceeb yog lub peev xwm los xaiv cov npe ntawm cov protein.
Sulfo-NHS ua haujlwm los ntawm kev cuam tshuam nrog cov amines tseem ceeb (piv txwv li -NH2 pawg) ntawm cov saw hlau ntawm lysine residues hauv cov protein. Qhov tseem ceeb, Sulfo-NHS sib xyaw ua ke "tag" cov protein, ua rau lawv yooj yim dua los txheeb xyuas thiab tshuaj xyuas hauv ntau yam kev sim. Qhov no tau ua rau ntau qhov chaw ntawm kev tshawb fawb muaj peev xwm txav mus rau pem hauv ntej nrog qhov tseeb dua thiab cov ntsiab lus siab dua.
Yog li, Sulfo-NHS siv rau dab tsi? Ib qho kev siv ntau ntawm cov tshuaj no yog nyob rau hauv kev tshawb fawb txog kev tiv thaiv kab mob. Sulfo-NHS tau pom tias muaj peev xwm sau cov tshuaj tiv thaiv kab mob thiab cov antigens tau zoo, qhib txoj hauv kev tshiab rau kev kawm txog cov kab mob thiab cov kab mob ntawm lub cev tiv thaiv kab mob. Tsis tas li ntawd,Sulfo-NHSsiv tau rau hauv kev tshawb fawb txog kev sib cuam tshuam ntawm cov protein thiab cov protein vim nws tso cai rau cov kws tshawb fawb kom paub sai thiab yooj yim thaum ob cov protein sib cuam tshuam.
Lwm qhov chaw uas Sulfo-NHS siv dav yog qhov ntawm proteomics. Proteomics kawm txog cov qauv thiab kev ua haujlwm ntawm txhua cov protein hauv ib lub cev, thiabSulfo-NHSyog ib qho cuab yeej tseem ceeb hauv qhov kev tshuaj xyuas no. Los ntawm kev cim cov protein nrog Sulfo-NHS, cov kws tshawb fawb tuaj yeem ua cov kev sim kom tau txais cov ntaub ntawv ntxaws ntxiv txog cov kab mob proteome, uas tom qab ntawd tuaj yeem pab txheeb xyuas cov cim qhia txog kab mob.
Sulfo-NHS kuj tseem ua lub luag haujlwm hauv kev tsim cov tshuaj tshiab. Thaum cov kws tshawb fawb tab tom sim tsim cov tshuaj tshiab, nws yog ib qho tseem ceeb kom paub tseeb tias nws tsom mus rau cov protein uas xav tau thiab tsis yog lwm cov protein hauv lub cev. Los ntawm kev sivSulfo-NHStxhawm rau xaiv cov cim protein, cov kws tshawb fawb tuaj yeem txheeb xyuas cov hom phiaj tseeb ntawm cov tshuaj muaj peev xwm, uas tuaj yeem pab ua kom cov txheej txheem tsim tshuaj sai dua.
Yog li ntawd koj muaj nws lawm! Sulfo-NHS tej zaum yuav tsis yog ib lo lus uas paub zoo sab nraum lub zej zog kev tshawb fawb, tab sis cov tshuaj no sai sai no ua ib qho cuab yeej muaj txiaj ntsig hauv kev tshawb fawb biomedical. Txij li kev tshawb fawb txog kev tiv thaiv kab mob mus rau proteomics mus rau kev tsim tshuaj, Sulfo-NHS tab tom pab cov kws tshawb fawb ua kom muaj kev vam meej loj hauv cov cheeb tsam no thiab peb zoo siab tos ntsoov pom cov kev tshawb pom dab tsi tom ntej.
Lub sijhawm tshaj tawm: Lub Rau Hli-12-2023