Ke hana nei ʻoe ma ke kahua o ka noiʻi biomedical? Inā pēlā, a laila ua lohe paha ʻoe iā Sulfo-NHS. I ka hoʻomau ʻia ʻana o ka ʻike ʻia ʻana o ke kuleana koʻikoʻi o kēia hui i ka noiʻi, ke komo nei kēia hui i nā keʻena hoʻokolohua he nui a puni ka honua. Ma kēia ʻatikala, kūkākūkā mākou i ke ʻano o Sulfo-NHS a no ke aha he mea hana waiwai nui ia no ka poʻe e aʻo ana i nā ʻepekema olaola.
ʻO ka mea mua, he aha ka Sulfo-NHS? He lōʻihi iki ka inoa, no laila e wehewehe kākou. ʻO Sulfo ke kū nei no ka waikawa sulfonic a ʻo NHS ke kū nei no N-hydroxysuccinimide. Ke hui pū ʻia kēia mau hui ʻelua,Sulfo-NHSua hana ʻia. He nui nā hoʻohana o kēia hui i ka noiʻi biomedical, akā ʻo kekahi o kona mau waiwai nui ka hiki ke koho i nā protein.
Hana ʻo Sulfo-NHS ma ka hana ʻana me nā amines mua (ʻo ia hoʻi nā hui -NH2) ma nā kaulahao ʻaoʻao o nā koena lysine i loko o nā protein. ʻO ke kumu, hoʻohui ʻo Sulfo-NHS i nā protein, e maʻalahi ai ka ʻike a me ke kālailai ʻana i nā ʻano hoʻokolohua like ʻole. Ua hopena kēia i nā wahi he nui o ka noiʻi e hiki ai ke neʻe i mua me ka pololei a me nā pae kikoʻī kiʻekiʻe.
No laila, he aha ka hoʻohana ʻia ʻana o Sulfo-NHS? ʻO kahi hoʻohana maʻamau o kēia hui ʻo ia ka noiʻi immunology. Ua hōʻike ʻia ʻo Sulfo-NHS e lepili pono i nā antibodies a me nā antigens, e wehe ana i nā ala hou no ke aʻo ʻana i nā maʻi a me nā maʻi o ka ʻōnaehana pale. Eia kekahi,Sulfo-NHShiki ke hoʻohana ʻia i nā haʻawina pilina protein-protein no ka mea e hiki ai i nā mea noiʻi ke ʻike koke a maʻalahi i ka wā e launa pū ai ʻelua mau protein.
ʻO kekahi wahi e hoʻohana nui ʻia ai ʻo Sulfo-NHS ʻo ia ka proteomics. Ke aʻo nei ʻo Proteomics i ke ʻano a me ka hana o nā protein āpau i loko o kahi meaola, aSulfo-NHShe mea hana koʻikoʻi ia i kēia loiloi. Ma ka hōʻailona ʻana i nā protein me Sulfo-NHS, hiki i nā mea noiʻi ke hana i nā hoʻokolohua e loaʻa ai ka ʻike kikoʻī e pili ana i ka proteome o kahi meaola i hāʻawi ʻia, a laila hiki ke kōkua i ka ʻike ʻana i nā biomarkers kūpono no ka maʻi.
He kuleana ko Sulfo-NHS i ka hoʻomohala ʻana i nā lāʻau lapaʻau hou. Ke hoʻāʻo nei nā mea noiʻi e hoʻomohala i kahi lāʻau lapaʻau hou, he mea nui e hōʻoia i ka kuhikuhi ʻana i ka protein i manaʻo ʻia a ʻaʻole i kekahi protein ʻē aʻe i loko o ke kino. Ma ka hoʻohana ʻanaSulfo-NHSno ke koho pono ʻana i nā protein, hiki i nā kānaka noiʻi ke ʻike i nā pahuhopu kikoʻī o nā lāʻau lapaʻau, hiki ke kōkua i ka wikiwiki o ke kaʻina hana hoʻomohala lāʻau.
Eia nō hoʻi! ʻAʻole paha he huaʻōlelo kaulana ʻo Sulfo-NHS ma waho o ke kaiāulu ʻepekema, akā ke lilo koke nei kēia hui i mea hana waiwai nui i ka noiʻi biomedical. Mai ka noiʻi immunology a i ka proteomics a i ka hoʻomohala ʻana i nā lāʻau lapaʻau, ke kōkua nei ʻo Sulfo-NHS i nā mea noiʻi e hana i nā holomua nui ma kēia mau wahi a hauʻoli mākou e ʻike i nā ʻike e hiki mai ana.
Ka manawa hoʻouna: Iune-12-2023